Intracellular protein scaffold-mediated display of random peptide libraries for phenotypic screens in mammalian cells
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چکیده
منابع مشابه
The maltose-binding protein as a scaffold for monovalent display of peptides derived from phage libraries.
Random peptide libraries are displayed on filamentous bacteriophage as fusions to either the minor coat protein, pIII, or the major coat protein, pVIII. We have devised a means of isolating the peptide displayed on a phage clone by transferring it to the N-terminus of the maltose-binding protein (MBP) of Escherichia coli encoded by malE. Transfer of a peptide sequence to monomeric MBP eliminate...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2001
ISSN: 1074-5521
DOI: 10.1016/s1074-5521(01)00031-x